All compounds entering the EU-OPENSCREEN ERIC compound collection are carefully characterised and annotated for basic physico-chemical (e.g. solubility, light absorbance, autofluorescence) and biological properties (e.g. cytotoxicity, antibiotic resistance) by testing in a standard panel of assays. Bioprofiling assays serve several purposes: (i) to identify and flag compounds with properties that could perturb specific bioassay read-out technologies (e.g. light quenching, luciferase inhibition, toxicity) in order to reduce false-positive results, therefore (ii) to secure reliability of screening results and thus, trusted values in the database; and (iii) to provide initial information about promising biological activities.
Compounds from the EU-OPENSCREEN compound collection will be tested in a standardised and automated manner to collect quantitative data on these activities. The tests are carried out by accredited screening partner sites in ERIC member countries. EU-OPENSCREEN’s network of screening sites offers our scientific collaborators access to a wide range of read-out technologies and implement formats for biochemical, biophysical and cell-based assays. The screening platforms adhere to high quality operating standards which are monitored by EU-OPENSCREEN. This will ensure that results generated in project-specific and bioprofiling screening campaigns are reliable, reproducible and comparable, thereby helping to meet the objective of driving standards in the conduct of chemical biology research.