Projects

Translocation Transfer

Alba Silipo, Antonie Molinaro

Affiliation:

Department of Chemical Sciences, University of Napoli Federico II, Naples, Italy

Current position:

Professor of Organic Chemistry

Contact:

Email: silipo@unina.it
Website: orcid.org/0000-0002-5394-6532 

Key words:

Bacterial cell envelope; lipopolysaccharides; peptidoglycan; microbial glycoconjugates; Structure-Bioactivity Relationship; structural biology; NMR; Carbohydrates

Expertise:

Bacterial cell envelope structure and function- Isolation, purification and structural characterization of microbial cell envelope glycoconjugates; study of their structure to function relationship. Conformational analysis of glycoconjugates by NMR and computational methods. Study of the protein-ligand interaction by NMR and computational methods. Structural elucidation of microbial polysaccharides and glycoconjugates from different sources (opportunistic, plant- and human-associated, extremophiles). Determination of the structure and function of Gram negative bacteria cell envelope components as lipopolysaccharides, PGN..; definition of their immunological perception by the host. Structural remodeling of microbial glycoconjugates to i) enable the bacterial adaptation to external environment and to ii) escape of the bacterium from the host immune surveillance during the its intra-cellular life-style. Role of the LPS in the symbiotic process involving Bradirhizobium and Leguminosae plants. Study of the molecular mechanisms at the basis of protein-carbohydrate interaction at host-pathogen interface.

Role in the network:

Provide spectroscopic, physical chemical and computational techniques (as ligand-based NMR spectroscopy, Molecular Mechanics and Dynamics calculations,docking/ CORCEMA-ST methods) to study the molecular mechanisms at the basis of protein-carbohydrate interaction (at host-pathogen interface.) and the conformational features of ligands when interacting with proteins.

References:

  • Belin BJ, Busset N, Giraud E, Molinaro A, Silipo A*, Newman DK*. Hopanoid lipids: from membranes to plant-bacteria interactions. Nat Rev Microbiol. 2018, doi: 10.1038/nrmicro.2017.173. volume 16, pages 304–315 (2018)
  • A. Silipo*, G. Vitiello, D. Gully, L. Sturiale, C. Chaintreuil, J. Fardoux, D. Gargani, H-I Lee, G. Kulkarni, N. Busset, R. Marchetti, A. Palmigiano, H. Moll, R. Engel, R. Lanzetta, L. Paduano, M. Parrilli, W-S Chang, O. Holst, D.K. Newman, D. Garozzo, G. D’Errico, E. Giraud & A. Molinaro (2014) Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes, Nature Communications, 5, Article number:5106, doi:10.1038/ncomms6106
  • Squeglia F, Marchetti R, Ruggiero A, Lanzetta R, Marasco D, Dworkin J, Petoukhov M, Molinaro A, Berisio R, Silipo A.* Chemical Basis of Peptidoglycan Discrimination by PrkC, a Key Kinase Involved in Bacterial Resuscitation from Dormancy. J. Am. Chem. Soc. 2011, 133, 20676-20679
  • Paciello*, A. Silipo*, L. Lembo-Fazio, L. Curcurù, A. Zumsteg, G. Noël, V. Ciancarella, L. Sturiale, A. Molinaro & M. L. Bernardini, (2013) Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation, PNAS; 110(46): E4345-54 * equal contribution
  • Silipo A, Leone MR, Erbs G, Lanzetta R, Parrilli M, Chang WS, Newman MA, Molinaro A. A Unique Bicyclic Monosaccharide from the Bradyrhizobium Lipopolysaccharide and Its Role in the Molecular Interaction with Plants. Angew Chem Int Ed Engl. 2011, Vol 50 Issue 52, 12610-12612