Projects

Translocation Transfer

Gaetan L.A Mislin

Affiliation:

University of Strasbourg - CNRS ESBS
300, Bld Sébastien Brant
CS 10413
67412 Illkirch
France

Contact:

Phone: +33 6 68 85 47 27
Email: mislin@unistra.fr
Webpage: http://bsc.unistra.fr/en/research-teams/team-schalkmislin/presentation/

Current Position:

Director of Research at the CNRS, co-Head of the team “Metals and Microorganisms : Biology, Chemistry and Applications”

Key words:

siderophore, antibiotic, metalloantibiotic, Trojan Horse strategy, vectorization, ICT iron chelators

Research profile:

  • Characterization of new siderophores
  • Total synthesis of natural siderophores
  • Synthesis of siderophore vectors dedicated to antibiotic Trojan Horse strategy
  • Synthesis of siderophore-related molecular tools (fluorescent siderophore, deuterium tagged siderophores).
  • Development of metalloantibiotics
  • Development of siderophore-inspired iron chelators devoted to Iron Chelation Therapy (ICT)

Expertise:

- Purification and structural characterization of bacterial siderophore - Organic and Medicinal Chemistry - Aromatic and Heterocyclic/Heteroaromatic synthesis - Microwave activated organic synthesis - Conjugation chemistry

Equipments:

  • Four bench/hood fully equipped for organic and medicinal chemistry
  • HPLC
  • Automated Flash chromatography
  • Microvawe reactor
  • Cryostat (20°C to -40°C)

References (10 most significant the last 5 years):

1-The complex of ferric-enterobactin with its transporter suggests a multi-step conformationally coupled process of uptake. Moynie, L.; Milenkovic, S.; Malloci, G; Gasser, V.; Baco, E.; Mislin, G.L.A.; Schalk, I.J.; Ceccarelli, M.; Naismith, J.H.* Nat. Comm. 2019, 10, 3673. 2- A key role for the periplasmic PfeE esterase in iron acquisition via the siderophore enterobactin in Pseudomonas aeruginosa. Perraud, Q.; Moynie, L.; Gasser, V.; Munier, M.; Godet, J.; Hoegy, F.; Mély, Y.; Mislin, G.L.A.; Naismith, J.; Schalk, I.J.* ACS Chem. Biol., 2018, 13, 2603-2614. 3- Bacterial Iron Uptake Pathways: Gates for the Import of Bactericide Compounds. Schalk, I.J.;* Mislin G.L.A. J. Med. Chem. 2017, 60, 4573–4576. (viewpoint) 4- Antipseudomonal Activity Enhancement of Luminescent Iridium(III) Dipyridylamine Complexes Under Visible Blue Light. E., Sauvageot; Elie, M.; Gaillard, S. *; Daniellou, R.; Fechter,P.; Schalk, I.J.; Gasser, V.; Renaud, J.-L.*; Mislin, G.L.A.* Metallomics 2017, 9, 1820-1827. 5- Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. Paulen, A.; Hoegy, F.; Roche, B.; Schalk, I.J., Mislin, G.L.A.* Bioorg. Med. Chem. Lett. 2017, 27, 4867-4870. 6-Catechol siderophores repress the pyochelin pathway and activate the enterobactin pathway in Pseudomonas aeruginosa: an opportunity for siderophore-antibiotic conjugates development. Gasser, V.; Baco, E.; Cunrath, O.; Saint-August, P.; Perraud, Q.; Zill, N.; Schleberger, C.; Schmidt, A.; Paulen, A.; Bumann, D.; Mislin G.L.A.; Schalk, I.J.* Environ. Microbiol. 2016, 18, 819-832. 7- Synthesis and antibiotic activity of oxazolidinone–catechol conjugates against Pseudomonas aeruginosa. Paulen, A.; Gasser, V.; Hoegy, F.; Perraud, Q.; Pesset, B.; Schalk, I.J.; Mislin G.L.A.* Org. Biomol. Chem. 2015, 13, 11567-11579. 8- Pseudomonas aeruginosa manipulates redox and iron homeostasis of its microbiota partner Aspergillus fumigatus via phenazines. Briard, B. ; Bomme, P. ; Lechner, B.E. ; Mislin, G.L.A., Lair, V. ; Prévost, M.- C.; Latge, J.-P. ; Haas, H. ; Beauvais, A.* Sci. Rep. 2015, 5:8220. 9- Diphenyl-benzo[1,3]dioxole-4-carboxylic acid pentafluorophenyl ester: a convenient catechol precursor in the synthesis of siderophore vectors suitable for antibiotic Trojan horse strategies. Baco, E.; Hoegy, F.; Schalk, I.J.; Mislin, G.L.A.* Org. Biomol. Chem. 2014, 12, 749-757. 10- Siderophore-dependent iron uptake systems as gates for antibiotic Trojan horse strategies against Pseudomonas aeruginosa. Mislin, G.L.A.; Schalk, I.J.* Metallomics 2014, 6, 408-420. (Review)