Projects

Translocation Transfer

Isabelle Schalk

Affiliation:

University of Strasbourg - CNRS ESBS
300, Bld Sébastien Brant
CS 10413
67412 Illkirch
France

Contact:

Phone: +33 6 68 85 47 19
Email: schalk@unistra.fr
Webpage: http://bsc.unistra.fr/en/research-teams/team-schalkmislin/presentation/

Current Position:

Director of Research at the CNRS, Head of the team “Metals and Microorgansims”

Key words:

siderophore, iron homesostasis, Pseudomonas, antiinfective drug discovery, antibiotic vectorisation

Research profile:

  • Investigation of iron homeostasis in Gram-negative bacteria and especially in P. aeruginosa.
  • Molecular interactions and mechanisms involved in iron acquisition in P. aeruginosa.
  • Investigate of the molecular mechanisms involved in the expression of the different iron uptake pathways and the complex network of regulation connecting all this different iron uptake pathways in P. aeruginosa.
  • Phenotypic adaptation of P. aeruginosa to different iron limited growth conditions (planktonic cultures, biofilms, eukaryotic infection assays, presence of siderophores, heme, etc …)
  • Vectorization of antibiotics into bacteria via siderophores (uptake assays, phenotypic adaptation of bacteria)

Expertise:

Molecular biology on P. aeruginosa, 55Fe uptake assays, MIC determination, proteomic, eukaryotic infection assay, siderophore biology, 25 years of expertise in iron homeostasis in P. aeruginosa

References (10 most significant the last 5 years):

  • Moynié L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, SCHALK IJ* , Ceccarelli M*, Naismith JH* (2019). The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model. Nature Commun. 10 : 3673.
  • Vigouroux A, Aumont-Nicaise M, Boussac A, Marty L, Lo Bello L, Legrand P, Brillet K, SCHALK IJ, Moréra S* (2019) A unique ferrous iron binding mode is associated with large conformational changes for the transport protein FpvC of Pseudomonas aeruginosa. FEBS J. doi: 10.1111/febs.15004.
  • Perraud Q, Moynié L, Gasser V, Munier M, Godet J, Hoegy F, Mély Y, Mislin GLA, Naismith JH, SCHALK IJ* (2018) A Key Role for the Periplasmic PfeE Esterase in Iron Acquisition via the Siderophore Enterobactin in Pseudomonas aeruginosa. ACS Chem Biol 13 : 2603-2614.
  • SCHALK IJ* (2018). Siderophore-antibiotic conjugates: exploiting iron uptake to deliver drugs into bacteria. Clin Microbiol Infect 24, 801-802. (Revue)
  • SCHALK IJ*(2018) A Trojan Horse strategy including a bacterial suicide action for the efficient use of a specific Gram-positive antibiotic on Gram-negative bacteria. J Med Chem. 61(9):3842-3844. (View point)
  • Ganne G, Brillet K, Basta B, Roche B, Hoegy F, Gasser V, SCHALK IJ* (2017). Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. ACS Chem Biol 12:1056-1065.
  • Paulen A, Hoegy F, Roche B, SCHALK IJ, Mislin GLA* (2017) Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. Bioorg. Med. Chem. Lett. 27: 4867-4870.
  • Gasser V, Baco E, Cunrath O, Saint-August P, Perraud Q, Zill N, Schleberger C, Schmidt A, Paulen A, Bumann D, Mislin GL, SCHALK IJ* (2016). Catechol siderophores repress the pyochelin pathway and activate the enterobactin pathway in Pseudomonas aeruginosa : an opportunity for siderophore-antibiotic conjugates development. Environ Microbiol 18:819-32.
  • Cunrath O, Geoffroy V, SCHALK IJ* (2016) Metallome of Pseudomonas aeruginosa: a role for siderophores. Environ Microbiol. 18(10):3258-3267
  • Cunrath O, Gasser V, Hoegy F, Reimmann C, Guillon L, SCHALK IJ* (2015). A cell biological view of the siderophore pyochelin iron uptake pathway in Pseudomonas aeruginosa. Environ Microbiol 17:171-185.