Projects

Translocation Transfer

Jean-Marie Pagès

Affiliation:

Aix-Marseille University,
France

Contact:

Phone : +33 491 32 46 97
Email : jean-marie.pages@univ-amu.fr

Key words:

Antibiotics, Bacterial membranes, gene regulation, porins, multidrug resistance, transport

Expertise:

The research unit is located in AMU Health Campus and comprises about 12 researchers (INSERM, CNRS, Univ, SSA) and several postdocs-PhDs. The team expertise covers various domains, from Bacterial membrane and transport (genetic and functional aspects, structural-activity relationship), Antibiotic resistance mechanisms (clinical involvement, regulation and prevalence), New antimicrobial compounds (new antibacterial activity or new vectorization strategy). Jean-Marie PAGES is author of over 250 publications and 4 patents. He has strong interest in (i) antibacterial agents with special concerns on antibiotic susceptibility and resistance in Gram-negative bacteria, (ii) Gram-negative bacterial membranes and, (iii) membrane transport (porins, efflux pumps). He has recently developed new concepts and methodologies, and created news tools to measure intra-bacterial accumulations of antibiotics and early steps of killing rates by antibacterial agents.

Recent references:

  • Masi et al. Mechanisms of envelope permeability and antibiotic influx and efflux in Gram-negative bacteria. Nat Microbiol. 2017 Feb 22;2:17001. doi: 10.1038/nmicrobiol.2017.1.
  • Nicolas-Chanoine et al. Interplay Between Membrane Permeability and Enzymatic Barrier Leads to Antibiotic-Dependent Resistance in Klebsiella Pneumoniae. Front Microbiol. 2018 Jun 29;9:1422. doi: 10.3389/fmicb.2018.01422.
  • Vergalli et al. Spectrofluorimetric quantification of antibiotic drug concentration in bacterial cells for the characterization of translocation across bacterial membranes. Nat Protoc. 2018 Jun;13(6):1348-1361. doi: 10.1038/nprot.2018.036.
  • Lôme et al. Multiparametric Profiling for Identification of Chemosensitizers against Gram-Negative Bacteria. Front Microbiol. 2018 Feb 19;9:204. doi: 10.3389/fmicb.2018.00204.
  • Dumont et al. Mechanistic aspects of maltotriose-conjugate translocation to the Gram-negative bacteria cytoplasm. Life Sci Alliance. 2018 Dec 28;2(1):e201800242. doi: 10.26508/lsa.201800242.
  • Dumont et al. Antibiotics and efflux: combined spectrofluorimetry and mass spectrometry to evaluate the involvement of concentration and efflux activity in antibiotic intracellular accumulation. J Antimicrob Chemother. 2019 Jan 1;74(1):58-65. doi: 10.1093/jac/dky396.
  • Davin-Regli et al. Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance. Clin Microbiol Rev. 2019 Jul 17;32(4). pii: e00002-19. doi: 10.1128/CMR.00002-19.
  • Vergalli et al. Porins and small-molecule translocation across the outer membrane of Gram- negative bacteria. Nat Rev Microbiol., 2019, in press.